The cost of the biocatalyst is one of the main bottlenecks in the industrial implementation of enzymes. The use of strategies such as one-step purification
and immobilization can reduce the costs of the downstream process, which contributes 50-80% of the total production cost (1). Furthermore, enzyme
immobilization allows to easily recovery and re-used, or applied in continuous processes allowing process intensification while their stability towards
chemo-physical process conditions is improved.
New technologies of immobilization are emerging based on biomolecular scaffolds, such as cellulose binding modules (CBMs), that could be genetically
fused to enzymes to allow specific adsorption into cellulosic materials without the need of chemical functionalization (Fig. 1), allowing a more cost-
effective and eco-friendly strategy of immobilization (2).
In the current work, the assessment of different commercial cellulosic supports for the one-step purification/immobilization of a 5-Hydroxymethylfurfural
oxidase (HMFO) and its applications in the synthesis of a renewable building block, 2,5-Furandicarboxylic acid (FDCA), was carried out.