This webinar explored diverse co-creation strategies crucial for mobilizing stakeholders and overcoming challenges in fostering healthier soils in urban and industrial areas. It introduced an analytical matrix to facilitate delving into critical parameters to inform effective soil interventions and the establishment of healthy soil living labs. The discussion, drawing from European case studies, aimed to inform adaptive Lab strategies addressing Soil Mission’s objectives across diverse sites, land use, and ownerships, scales of action, levels of governance interactions, and the variability in multi-stakeholder cooperations (disciplines and geography). It also highlighted the need to consider forms of land optimization for ecological functions, the type of intermediation into knowledge co-production networks, and the legitimization of access to land for adaptive reuse and soil regeneration.