Artificial Intelligence (AI) is playing a crucial role both in the technological advances and emerging advanced threats in cybersecurity. Despite efforts by competent authorities in Europe to regulate the use of AI in a way that aligns with the ethics and individuals’ fundamental rights, there are still challenges to be tackled, not to mention the malicious use of AI by cybercriminals. In this paper we present a novel framework that is composed of innovative cybersecurity services that leverage AI to provide support in the management of the incident response and recovery lifecycle of the critical entities’ systems against advanced attacks. The paper describes the main components and architecture of the AI4CYBER framework and provides a clear understanding of the application of the autonomous intelligent cybersecurity services and their role in enforcing defensive actions throughout the entire lifecycle of the systems.