Published January 21, 2025 | Version v2
Dataset Open

A LexA-like repressor and global H-NS-like regulators enable the fine-tuning of R-tailocin expression in environmental Pseudomonas

  • 1. ROR icon University of Lausanne
  • 2. Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland

Description

This dataset is related to "A LexA-like repressor and global H-NS-like regulators enable the fine-tuning of R-tailocin expression in environmental Pseudomonas" and contains :

  • "RNA-seq_analyzed_dataset.xlsx": The processed RNA-seq data (related to Fig 1, Fig 3 and S7 Fig). 
  • "Expression_data_analyzed.xlsx": The processed expression data (related to Fig 2, Fig 3, S2 Fig, S3 Fig and S9 Fig).
  • "Chip_seq_CHA0_I_CPM_normalized.bw": Bigwig file used for IGV visualization for wild type Pseudomonas protegens CHA0 under inducing conditions (related to Fig 2, Fig 3, S5 Fig, S6 Fig, S10 Fig, S11 Fig, S12 Fig, S13 Fig, S14 Fig and S15 Fig; reference genome: P. protegens CHA0 LS999205.1).
  • "Chip_seq_CHA0_NI_CPM_normalized.bw": Bigwig file used for IGV visualization for wild type P. protegens CHA0 under non-inducing conditions (related to Fig 2, Fig 3, S5 Fig, S6 Fig, S10 Fig, S11 Fig, S12 Fig, S13 Fig, S14 Fig and S15 Fig; reference genome: P. protegens CHA0 LS999205.1).
  • "Chip_seq_prtR_V5_I_CPM_normalized.bw": Bigwig file used for IGV visualization for the P. protegens CHA0 PrtR-V5 under inducing conditions (related to Fig 2, S6 Fig, S12 Fig, S13 Fig, S14 Fig and S15 Fig; reference genome: P. protegens CHA0 LS999205.1).
  • "Chip_seq_prtR_V5_NI_CPM_normalized.bw": Bigwig file used for IGV visualization for P. protegens CHA0 PrtR-V5 under non-inducing conditions (related to Fig 2, S6 Fig, S12 Fig, S13 Fig, S14 Fig and S15 Fig; reference genome: P. protegens CHA0 LS999205.1).
  • "Chip_seq_mvaT_V5_I_CPM_normalized.bw": Bigwig file used for IGV visualization for P. protegens CHA0 MvaT-V5 under inducing conditions (related to Fig 3, S6 Fig, S10 Fig, S11 Fig, S12 Fig, S13 Fig, S14 Fig and S15 Fig; reference genome: P. protegens CHA0 LS999205.1).
  • "Chip_seq_mvaT_V5_NI_CPM_normalized.bw": Bigwig file used for IGV visualization for P. protegens CHA0 MvaT-V5 under non-inducing conditions (related to Fig 3, S6 Fig, S10 Fig, S11 Fig, S12 Fig, S13 Fig, S14 Fig and S15 Fig; reference genome: P. protegens CHA0 LS999205.1).
  • "Chip_seq_mvaV_V5_I_CPM_normalized.bw": Bigwig file used for IGV visualization for P. protegens CHA0 MvaV-V5 under inducing conditions (related to Fig 3, S6 Fig, S10 Fig, S11 Fig, S12 Fig, S13 Fig, S14 Fig and S15 Fig; reference genome: P. protegens CHA0 LS999205.1).
  • "Chip_seq_mvaV_V5_NI_CPM_normalized.bw": Bigwig file used for IGV visualization for P. protegens CHA0 MvaV-V5 under non-inducing conditions (related to Fig 3, S6 Fig, S10 Fig, S11 Fig, S12 Fig, S13 Fig, S14 Fig and S15 Fig; reference genome: P. protegens CHA0 LS999205.1).
  • "GC_content.csv": Percentage of GC content in the different peaks identified in the ChIP-seq (related to S11 Fig).
  • "mvaT_mvaV_differences.xlsx": The processed data for the differences found in the RNA-seq between the double mutant ΔmvaTΔmvaV and wild type P. protegens CHA0 in non-induced condition and the double mutant ΔmvaTΔmvaV andwild type P. protegens CHA0 in induced condition (related to Fig 3).
  • "S1_Fig_Expression.xlsx": The processed expression data (related to S1 Fig).
  • "S1_Fig_CFU.xlsx": The colony forming units obtained following exposure to the two inducing agents (mitomycin C and hydrogen peroxide; related to S1 Fig)
  • "S4_Fig.xlsx": The processed growth curve data for the mutants used for the ChIP-seq (related to S4 Fig).
  • "Chip_seq_parB_V5_I_CPM_normalized.bw": Bigwig file used for IGV visualization for P. protegens CHA0 ParB-V5 under inducing conditions (related to S5 Fig and S6 Fig; reference genome: P. protegens CHA0 LS999205.1).
  • "Chip_seq_parB_V5_NI_CPM_normalized.bw": Bigwig file used for IGV visualization for P. protegens CHA0 ParB-V5 under non-inducing conditions (related to S5 Fig and S6 Fig; reference genome: P. protegens CHA0 LS999205.1).
  • "S7_Fig_mvaT_mvaV_differences.xlsx": The processed data for the differences found in the RNA-seq between the single mutants ΔmvaT or ΔmvaV and wild type P. protegens CHA0 in non-induced condition and the single mutants ΔmvaT or ΔmvaV and wild type P. protegens CHA0 in induced condition (related to S7 Fig).

Files

GC_content.csv

Files (16.5 MB)

Name Size Download all
md5:cfb353142d0334acfe27af0b559d3542
814.3 kB Download
md5:62743e6ff03326ffbc398257e9dcd3cf
841.2 kB Download
md5:b2be91b63b93252d2e14aa901012f870
963.7 kB Download
md5:2a278ef4763c696b87319c7974954c2c
946.5 kB Download
md5:7e552b277859a01ee5c2a4e0bb99ab79
934.1 kB Download
md5:9be123c8d5500a8c7781672aadb7f840
906.9 kB Download
md5:38f8dfce525400c6ac952b230241b447
936.4 kB Download
md5:0c8d756c05f6bc8c75b951a4877ceb89
944.7 kB Download
md5:fdb2a8a3526d17cac55eea75f413309c
949.4 kB Download
md5:22856fd20972d2dd0ec5ee3bed0b3ca6
898.3 kB Download
md5:ec63591ea85076abb106caef47efd869
945.1 kB Download
md5:92425458a8c26b86170b16c77f9ca760
20.5 kB Preview Download
md5:59c5a11ee29aac193132b2bd14b70d4a
145.1 kB Download
md5:87397542bbcb08dd335d396a6575f9f5
5.0 MB Download
md5:565c6abea0fabbd47d6f01a82b5e5ad7
21.5 kB Download
md5:4bcdcd520551dd8fb5ac2e4e435b7218
362.9 kB Download
md5:28fb56db5c2a51d0fa77122f1e5fc768
68.8 kB Download
md5:e0445fef6df300b57fe51d41db2262ab
800.1 kB Download

Additional details

Funding

Swiss National Science Foundation
Towards building microbial consortia for pest control: exploring adaptation to insect hosts and intraspecific diversity of pathogenicity and competition traits in insecticidal pseudomonads 184666
Swiss National Science Foundation
NCCR Microbiomes (phase I) 180575
Swiss National Science Foundation
NCCR Microbiomes (phase II) 225148