Published January 21, 2025
| Version v2
Dataset
Open
A LexA-like repressor and global H-NS-like regulators enable the fine-tuning of R-tailocin expression in environmental Pseudomonas
Creators
Description
This dataset is related to "A LexA-like repressor and global H-NS-like regulators enable the fine-tuning of R-tailocin expression in environmental Pseudomonas" and contains :
- "RNA-seq_analyzed_dataset.xlsx": The processed RNA-seq data (related to Fig 1, Fig 3 and S7 Fig).
- "Expression_data_analyzed.xlsx": The processed expression data (related to Fig 2, Fig 3, S2 Fig, S3 Fig and S9 Fig).
- "Chip_seq_CHA0_I_CPM_normalized.bw": Bigwig file used for IGV visualization for wild type Pseudomonas protegens CHA0 under inducing conditions (related to Fig 2, Fig 3, S5 Fig, S6 Fig, S10 Fig, S11 Fig, S12 Fig, S13 Fig, S14 Fig and S15 Fig; reference genome: P. protegens CHA0 LS999205.1).
- "Chip_seq_CHA0_NI_CPM_normalized.bw": Bigwig file used for IGV visualization for wild type P. protegens CHA0 under non-inducing conditions (related to Fig 2, Fig 3, S5 Fig, S6 Fig, S10 Fig, S11 Fig, S12 Fig, S13 Fig, S14 Fig and S15 Fig; reference genome: P. protegens CHA0 LS999205.1).
- "Chip_seq_prtR_V5_I_CPM_normalized.bw": Bigwig file used for IGV visualization for the P. protegens CHA0 PrtR-V5 under inducing conditions (related to Fig 2, S6 Fig, S12 Fig, S13 Fig, S14 Fig and S15 Fig; reference genome: P. protegens CHA0 LS999205.1).
- "Chip_seq_prtR_V5_NI_CPM_normalized.bw": Bigwig file used for IGV visualization for P. protegens CHA0 PrtR-V5 under non-inducing conditions (related to Fig 2, S6 Fig, S12 Fig, S13 Fig, S14 Fig and S15 Fig; reference genome: P. protegens CHA0 LS999205.1).
- "Chip_seq_mvaT_V5_I_CPM_normalized.bw": Bigwig file used for IGV visualization for P. protegens CHA0 MvaT-V5 under inducing conditions (related to Fig 3, S6 Fig, S10 Fig, S11 Fig, S12 Fig, S13 Fig, S14 Fig and S15 Fig; reference genome: P. protegens CHA0 LS999205.1).
- "Chip_seq_mvaT_V5_NI_CPM_normalized.bw": Bigwig file used for IGV visualization for P. protegens CHA0 MvaT-V5 under non-inducing conditions (related to Fig 3, S6 Fig, S10 Fig, S11 Fig, S12 Fig, S13 Fig, S14 Fig and S15 Fig; reference genome: P. protegens CHA0 LS999205.1).
- "Chip_seq_mvaV_V5_I_CPM_normalized.bw": Bigwig file used for IGV visualization for P. protegens CHA0 MvaV-V5 under inducing conditions (related to Fig 3, S6 Fig, S10 Fig, S11 Fig, S12 Fig, S13 Fig, S14 Fig and S15 Fig; reference genome: P. protegens CHA0 LS999205.1).
- "Chip_seq_mvaV_V5_NI_CPM_normalized.bw": Bigwig file used for IGV visualization for P. protegens CHA0 MvaV-V5 under non-inducing conditions (related to Fig 3, S6 Fig, S10 Fig, S11 Fig, S12 Fig, S13 Fig, S14 Fig and S15 Fig; reference genome: P. protegens CHA0 LS999205.1).
- "GC_content.csv": Percentage of GC content in the different peaks identified in the ChIP-seq (related to S11 Fig).
- "mvaT_mvaV_differences.xlsx": The processed data for the differences found in the RNA-seq between the double mutant ΔmvaTΔmvaV and wild type P. protegens CHA0 in non-induced condition and the double mutant ΔmvaTΔmvaV andwild type P. protegens CHA0 in induced condition (related to Fig 3).
- "S1_Fig_Expression.xlsx": The processed expression data (related to S1 Fig).
- "S1_Fig_CFU.xlsx": The colony forming units obtained following exposure to the two inducing agents (mitomycin C and hydrogen peroxide; related to S1 Fig)
- "S4_Fig.xlsx": The processed growth curve data for the mutants used for the ChIP-seq (related to S4 Fig).
- "Chip_seq_parB_V5_I_CPM_normalized.bw": Bigwig file used for IGV visualization for P. protegens CHA0 ParB-V5 under inducing conditions (related to S5 Fig and S6 Fig; reference genome: P. protegens CHA0 LS999205.1).
- "Chip_seq_parB_V5_NI_CPM_normalized.bw": Bigwig file used for IGV visualization for P. protegens CHA0 ParB-V5 under non-inducing conditions (related to S5 Fig and S6 Fig; reference genome: P. protegens CHA0 LS999205.1).
- "S7_Fig_mvaT_mvaV_differences.xlsx": The processed data for the differences found in the RNA-seq between the single mutants ΔmvaT or ΔmvaV and wild type P. protegens CHA0 in non-induced condition and the single mutants ΔmvaT or ΔmvaV and wild type P. protegens CHA0 in induced condition (related to S7 Fig).
Files
GC_content.csv
Files
(16.5 MB)
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Additional details
Funding
- Swiss National Science Foundation
- Towards building microbial consortia for pest control: exploring adaptation to insect hosts and intraspecific diversity of pathogenicity and competition traits in insecticidal pseudomonads 184666
- Swiss National Science Foundation
- NCCR Microbiomes (phase I) 180575
- Swiss National Science Foundation
- NCCR Microbiomes (phase II) 225148