Fig. 1 in In silico characterization of class II plant defensins from Arabidopsis thaliana

Fig. 1. Flowchart of identification of class II defensins. The indigo boxes indicated steps performed by Perl scripts and the red boxes, steps curated by hand. The white boxes indicated the number of sequences for each respective step. The sequences from A. thaliana were retrieved from UniProt database; the defensin RegEx "CX2-18CX3CX2-10[GAPSIDERYW]XCX4-17CXC" was determined by Zhu (Zhu, 2008). The complete sequences were retrieved according the UniProt annotations; and the sequences predicted to be secreted were selected according to the Phobius prediction, presenting signal peptide and no transmembrane domains. The selected sequences at the end of the process represented less than 1% of all defensin sequences from A. thaliana. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)