Fig. 2. A in Phenolic fingerprints of the Pacific seagrass Phyllospadix torreyi - Structural characterization and quantification of undescribed flavonoid sulfates

Fig. 2. A - Typical HPLC profile of a Phyllospadix torreyi crude extract (recorded at 330 nm). Peak number, retention time (min), assignment. 1: 12.1, caffeic acid; 2: 13.3, nepetin 7,4' -disulfate; 3: 13.9, 5-methoxyluteolin 7,3′-disulfate; 4: 14.3, 6-hydroxyluteolin 7,3′-disulfate; 5: 16.7, coumaric acid; 6: 17.8, luteolin 7,3′-disulfate; 7: 18.7, nepetin 7,3′- disulfate; 8: 19.2, 5-methoxyluteolin 7-sulfate; 9: 19.9, 6-hydroxyluteolin 7-sulfate; 10: 23.2, rosmarinic acid; 11: 23.7, luteolin 7-sulfate; 12: 24.3, nepetin 7-sulfate; 13: 25.6, luteolin 3′-sulfate; 14: 26.7, nepetin 3′-sulfate; 15: 26.9, hispidulin 7-sulfate; 16: 27.6, jaceosidin 7-sulfate. B - HPLC profile after acid hydrolysis. Peak denomination, retention time (min), assignment. 1: 12.1, caffeic acid; 5: 16.7, coumaric acid; 17: 22.9, traces of 6-hydroxyluteolin 3′-sulfate; 10: 23.2, rosmarinic acid; a: 24.1, 6- hydroxyluteolin; b: 25.1, 5-methoxyluteolin; c: 30.2, luteolin; d: 30.9, nepetin; e: 33.6, hispidulin; f: 34.0, jaceosidin. All numbers and denominations correspond to compounds described in the results section and in Table 1 and Fig. 3.