Published December 21, 2022 | Version 1
Technical note Open

Purification of SLC25A37 from Expi293FTM Cells

  • 1. Centre for Medicines Discovery, University of Oxford. Oxford, UK

Description

This protocol is used to purify the SLC transporter expressed in Expi293FTM Cells using detergent solubilization followed by a series of different chromatographic methods. Depending on the nature of the expression construct and experimental requirements, the GFP tag can be removed using a proteolytic reaction.

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SP001G-Y_SLC25A37 Purification.pdf

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Additional details

Funding

European Commission
ReSOLUTE – Research empowerment on solute carriers (ReSOLUTE) 777372