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Published September 27, 2022 | Version 0.1
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Datasets for napari-lattice: Lattice Lightsheet Analysis

  • 1. Walter and Eliza Hall Institute of Medical Rsesearch

Description

Sample data acquired using the Zeiss lattice lightsheet microscope. The images are of human red blood cells acquired in the 488 channel. This dataset is associated with the software napari-lattice, which is a plugin for napari, an n-dimensional viewer in Python.

Dimensions are in the format (Time, Channel, Z, Y, X)

  • RBC_full_one_timepoint.czi: (1, 1,  834, 300, 2048)
  • RBC_full_time_series.czi: (3, 1,  834, 300, 2048)
  • RBC_medium_LLSZ.czi: (5, 1,  834, 297, 279)
  • RBC_tiny.czi:  (1, 1,  834, 118, 209)

Voxel sizes in z, y and x respectively: ( 0.3, 0.1449922, 0.1449922)

 

napari-lattice can process lattice lightsheet datasets skewed in the X or Y direction, and you can also create full analysis workflows in napari. For more information: 

https://github.com/BioimageAnalysisCoreWEHI/napari_lattice

 

Files

Files (4.9 GB)

Name Size Download all
md5:31e59fb70e68dc432dbd3ffe4314f3ff
1.0 GB Download
md5:f4f11041b3c444962c3bb263cf09f0a3
3.1 GB Download
md5:9cb195ee9a303ad776da1d7e585ffe9d
703.2 MB Download
md5:e1b7f9a88b0008d89a9ce07c4b7a48fe
48.9 MB Download

Additional details

References

  • Geoghegan, N.D., Evelyn, C., Whitehead, L.W. et al. 4D analysis of malaria parasite invasion offers insights into erythrocyte membrane remodeling and parasitophorous vacuole formation. Nat Commun 12, 3620 (2021). https://doi.org/10.1038/s41467-021-23626-7