Datasets for napari-lattice: Lattice Lightsheet Analysis
- 1. Walter and Eliza Hall Institute of Medical Rsesearch
Description
Sample data acquired using the Zeiss lattice lightsheet microscope. The images are of human red blood cells acquired in the 488 channel. This dataset is associated with the software napari-lattice, which is a plugin for napari, an n-dimensional viewer in Python.
Dimensions are in the format (Time, Channel, Z, Y, X)
- RBC_full_one_timepoint.czi: (1, 1, 834, 300, 2048)
- RBC_full_time_series.czi: (3, 1, 834, 300, 2048)
- RBC_medium_LLSZ.czi: (5, 1, 834, 297, 279)
- RBC_tiny.czi: (1, 1, 834, 118, 209)
Voxel sizes in z, y and x respectively: ( 0.3, 0.1449922, 0.1449922)
We have updated the dataset with pointspread functions (PSFs) for each channel. These are Zeiss simulated ones. There is a czi version and a tiff version.
If you would like more PSFs, visit this comprehensive repo from EPFL: https://zenodo.org/records/14505724
napari-lattice can process lattice lightsheet datasets from Zeiss Lattice lightsheet 7. We have updated the interface and API. Visit:
https://github.com/BioimageAnalysisCoreWEHI/napari_lattice
Files
PSFs_zeiss_simulated_czi.zip
Files
(4.9 GB)
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Additional details
References
- Geoghegan, N.D., Evelyn, C., Whitehead, L.W. et al. 4D analysis of malaria parasite invasion offers insights into erythrocyte membrane remodeling and parasitophorous vacuole formation. Nat Commun 12, 3620 (2021). https://doi.org/10.1038/s41467-021-23626-7