Influence of anatomical features of different brain regions on the spatial localization of fiber photometry signals
Creators
- 1. Istituto Italiano di Tecnologia (IIT), Center for Biomolecular Nanotechnologies
- 2. Istituto Italiano di Tecnologia (IIT), Center for Biomolecular Nanotechnologies & Dipartimento di Ingegneria dell'Innovazione, Università del Salento
- 3. Dipartimento di Scienze e Tecnologie Biologiche e Ambientali, Università del Salento
Description
ABSTRACT: Fiber photometry is widely used in neuroscience labs for in vivo detection of functional fluorescence from optical indicators of neuronal activity with a simple optical fiber. The fiber is commonly placed next to the region of interest to both excite and collect the fluorescence signal. However, the path of both excitation and fluorescence photons is altered by the uneven optical properties of the brain, due to local variation of the refractive index, different cellular types, densities and shapes. Nonetheless, the effect of the local anatomy on the actual shape and extent of the volume of tissue that interfaces with the fiber has received little attention so far. To fill this gap, we measured the size and shape of fiber photometry efficiency field in the primary motor and somatosensory cortex, in the hippocampus and in the striatum of the mouse brain, highlighting how their substructures determine the detected signal and the depth at which photons can be mined. Importantly, we show that the information on the spatial expression of the fluorescent probes alone is not sufficient to account for the contribution of local subregions to the overall collected signal, and it must be combined with the optical properties of the tissue adjacent to the fiber tip.
Files
Data_Montinaro_BOE_FiberPhotometry.zip
Additional details
Funding
- MODEM – Multipoint Optical DEvices for Minimally invasive neural circuits interface 677683
- European Commission
- BrainBIT – All-optical brain-to-brain behaviour and information transfer 692943
- European Commission
- DEEPER – DEEP BRAIN PHOTONIC TOOLS FOR CELL-TYPE SPECIFIC TARGETING OF NEURAL DISEASES 101016787
- European Commission
- Controlling the spatial extent of light-based monitoring and manipulation of neural activity in vivo 1UF1NS108177-01
- National Institutes of Health
- NanoBRIGHT – BRInGing nano-pHoTonics into the brain 828972
- European Commission