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Published July 14, 2021 | Version v1
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Phosphomapping of S. cerevisiae Pkc1 in response to hydroxyurea treatment

  • 1. Mass Spectrometry Facility, Max Perutz Labs, University of Vienna, Vienna BioCenter (VBC), Dr. Bohr-Gasse 9, 1030 Vienna, Austria
  • 2. Center for Medical Biochemistry, Max Perutz Labs, Medical University of Vienna, Vienna BioCenter (VBC), Dr. Bohr-Gasse 9, 1030 Vienna, Austria
  • 3. Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston, MA 02118, United States
  • 4. Department of Biochemistry and Cell Biology, Max Perutz Labs, University of Vienna, Vienna BioCenter (VBC), Dr. Bohr-Gasse 9, 1030 Vienna, Austria

Description

We performed phosphorylation site mapping on of budding yeast Pkc1 applying a quantitative mass spectrometry (MS)-based proteomics approach based on stable isotope labeling with amino acids in cell culture (SILAC). We determined changes in the Pkc1 phosphorylation pattern in response to genotoxic stress induced by Hydroxyurea treatment. This dataset includes all MS RAW files.

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mq_Phospho (STY)Sites.txt

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