Published March 15, 2016
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Figure 5 in Chemistry supports the identification of gender-specific reproductive tissue in Tyrannosaurus rex
- 1. Department of Biological Sciences, North Carolina State University, Raleigh NC 27695, USA. North Carolina Museum of Natural Sciences, Raleigh NC 27601, USA
- 2. Department of Biological Sciences, North Carolina State University, Raleigh NC 27695, USA.
- 3. Department of Anatomy, Des Moines University, Des Moines IA 50312, USA.
- 4. Department of Agrobiology, Niigata University, Niigata 9502181, Japan.
Description
Figure 5. Immunochemical staining of bone using monoclonal antibodies raised against the sulfated glycosaminoglycan keratan sulfate. (A,C,E,G,I,K,M,O) are overlay images showing tissue and localized binding; (B,D,F,H,},L,N,P) are fluorescent images using FITC label. Chicken CB (A,B) shows no binding; chicken MB (C,D) shows positive staining, with green fluorescent signal representing antibody-antigen complexes, arranged in globular clusters. Similarly, ostrich femoral CB (E,F) does not bind antibodies, but ostrich MB (G,H) is positive for binding using the same data collection parameters. T. rex CB (I,}), does not show evidence of localized antibody binding, but sections of isolated MB (K,L) show localized specific binding to antibodies in a globular pattern, as seen in the chicken. (M,N) cortical region of tarsometatarsus and (O,P) internal (medullary) region of chicken genetically diagnosed with avian osteopetrosis (Materials and Methods) exposed to anti-keratan sulfate antibodies. No binding is seen, using same data collection parameters.
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