Optimisation of HTT-HAP40 purification using heparin affinity chromatography - 2019/01/29
Project: Biophysical investigation of purified HTT protein samples
Experiment: Optimisation of HTT-HAP40 purification using heparin affinity chromatography
Date completed: 2019/01/29
Rationale: The current protocol for HTT and HTT-HAP40 purification I am using requires a long incubation of clarified cell lysate with FLAG resin. To potentially improve yields and sample quality, it would perhaps be beneficial to have a quick heparin resin purification step prior to FLAG binding which may also remove contaminating nucleic acid material. To test this hypothesis, small-scale purification of Q23 HTT-HAP40 samples in different buffer systems were conducted using heparin and FLAG affinity chromatography which showed the sample bound heparin resin – see https://zenodo.org/record/2553669. Now this need to be scaled up and tested more stringently.
Optimisation of HTT-HAP40 purification using heparin affinity chromatography 20190129.pdf
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