Dataset Open Access

Optimisation of HTT-HAP40 purification using heparin affinity chromatography (2019/01/14)

Harding, Rachel; Hutchinson, Ashley; Seitova, Alma; Arrowsmith, Cheryl; Edwards, Aled

Project - Huntingtin structure-function open lab notebook.  

Experiment - Optimisation of HTT-HAP40 purification using heparin affinity chromatography.

Aims - The current protocol for HTT and HTT-HAP40 purification I am using requires a long incubation of clarified cell lysate with FLAG resin. To potentially improve yields and sample quality, it would perhaps be beneficial to have a quick heparin resin purification step prior to FLAG binding which may also remove contaminating nucleic acid material. To test this hypothesis, small-scale purification of Q23 HTT-HAP40 samples in different buffer systems will be carried out using heparin and FLAG affinity chromatography. 

Dr. Harding is the recipient of the Huntington's Disease Society of America Berman Topper Career Development Fellowship which funds and supports this research. Dr. Harding's research is also supported with generous funding from the Huntington Society of Canada and the CHDI Foundation. The SGC is a registered charity (number 1097737) that receives funds from AbbVie, Bayer Pharma AG, Boehringer Ingelheim, Canada Foundation for Innovation, Eshelman Institute for Innovation, Genome Canada through Ontario Genomics Institute [OGI-055], Innovative Medicines Initiative (EU/EFPIA) [ULTRA-DD grant no. 115766], Janssen, Merck KGaA, Darmstadt, Germany, MSD, Novartis Pharma AG, Ontario Ministry of Research, Innovation and Science (MRIS), Pfizer, São Paulo Research Foundation-FAPESP, Takeda, and Wellcome.
Files (1.4 MB)
Name Size
Optimisation_HTT-HAP40_purification_Heparin_20190114.docx
md5:dbad5ac4b64da9f54239ced399ce8c4f 		996.9 kB Download
Optimisation_HTT-HAP40_purification_Heparin_20190114.pdf
md5:c8e1afadba7842661a0463bf606bc0f1 		359.6 kB Download
256
87
views
downloads
All versions This version
Views 256256
Downloads 8787
Data volume 42.8 MB42.8 MB
Unique views 230230
Unique downloads 6666
More info on how stats are collected.
Indexed in
Publication date:
January 14, 2019
DOI:
10.5281/zenodo.2553669

Zenodo DOI Badge

DOI 

10.5281/zenodo.2553669

Markdown 

[![DOI](https://zenodo.org/badge/DOI/10.5281/zenodo.2553669.svg)](https://doi.org/10.5281/zenodo.2553669)

reStructedText 

.. image:: https://zenodo.org/badge/DOI/10.5281/zenodo.2553669.svg
   :target: https://doi.org/10.5281/zenodo.2553669

HTML 

<a href="https://doi.org/10.5281/zenodo.2553669"><img src="https://zenodo.org/badge/DOI/10.5281/zenodo.2553669.svg" alt="DOI"></a>

Image URL 

https://zenodo.org/badge/DOI/10.5281/zenodo.2553669.svg

Target URL 

https://doi.org/10.5281/zenodo.2553669

Keyword(s):
Huntingtin HAP40 Protein purification Huntington's disease
Communities:
License (for files):
Creative Commons Attribution 4.0 International

Versions

Version 1 10.5281/zenodo.2553669 Jan 14, 2019
Cite all versions? You can cite all versions by using the DOI 10.5281/zenodo.2553668. This DOI represents all versions, and will always resolve to the latest one. Read more.

Share

Cite as

Export