Dataset Open Access
Harding, Rachel;
Hutchinson, Ashley;
Seitova, Alma;
Arrowsmith, Cheryl;
Edwards, Aled
Project - Huntingtin structure-function open lab notebook.
Experiment - Optimisation of HTT-HAP40 purification using heparin affinity chromatography.
Aims - The current protocol for HTT and HTT-HAP40 purification I am using requires a long incubation of clarified cell lysate with FLAG resin. To potentially improve yields and sample quality, it would perhaps be beneficial to have a quick heparin resin purification step prior to FLAG binding which may also remove contaminating nucleic acid material. To test this hypothesis, small-scale purification of Q23 HTT-HAP40 samples in different buffer systems will be carried out using heparin and FLAG affinity chromatography.
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Optimisation_HTT-HAP40_purification_Heparin_20190114.docx
md5:dbad5ac4b64da9f54239ced399ce8c4f |
996.9 kB | Download |
Optimisation_HTT-HAP40_purification_Heparin_20190114.pdf
md5:c8e1afadba7842661a0463bf606bc0f1 |
359.6 kB | Download |
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