FuturEnzyme: Technologies of the Future for Low-Cost Enzymes for Environment-Friendly Products

Part of EU Open Research Repository
Published September 19, 2023 | Version 1
Data paper Open

Sub-micro- and nano-sized polyethylene terephthalate deconstruction with engineered protein nanopores

Creators

  • 1. ROR icon Barcelona Supercomputing Center
  • 2. ROR icon Universidad Complutense de Madrid
  • 3. ROR icon Instituto de Catálisis y Petroleoquímica
  • 4. ROR icon Consejo Superior de Investigaciones Científicas
  • 5. CSIC, Consejo Superior de Investigaciones Cientificas
  • 6. CSIC
  • 7. Institute of Catalysis - CSIC
  • 8. ICREA -Institucio Catalana de Recerca i Estudis Avançats

Description

The identification or design of biocatalysts to mitigate the accumulation of plastics, including sub-micro- and nano-sized polyethylene terephthalate (nPET), is becoming a global challenge. Here we computationally incorporated two hydrolytic active sites with geometries similar to that of Idionella sakaiensis PET hydrolase, to fragaceatoxin C (FraC), a membrane pore-forming protein. FraCm1/m2 could be assembled into octameric nanopores (7.0 nm high × 1.6–6.0 nm entry), which deconstructed (40 °C, pH 7.0) nPET from GoodFellow, commodities and plastic bottles. FraCm1 and FraCm2 degrade nPET by endo- and exo-type chain scission. While FraCm1 produces bis(2-hydroxyethyl) terephthalate as the main product, FraCm2 yields a high diversity of oligomers and terephthalic acid. Mechanistic and biochemical differences with benchmark PET hydrolases, along with pore and nPET dynamics, suggest that these pore-forming protein catalytic nanoreactors do not deconstruct macro-PET but are promising in nanotechnology for filtering, capturing and breaking down nPET, for example, in wastewater treatment plants.

Files

Raw MS data Supp 14_NatCal2023.zip

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Additional details

Related works

Is supplement to
Journal article: 10.1038/s41929-023-01048-6 (DOI)

Funding

European Commission
FuturEnzyme - Technologies of the Future for Low-Cost Enzymes for Environment-Friendly Products 101000327
European Commission
NYMPHE - New system-driven bioremediation of polluted habitats and environment 101060625
European Commission
PlasticsFatE - Plastics fate and effects in the human body 965367