Miniaturized, high numerical aperture confocal fluorescence detection enhanced with pyroelectric droplet accumulation for sub-attomole analyte diagnosis
Creators
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1.
VTT Technical Research Centre of Finland
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2.
Institute of Applied Science and Intelligent Systems
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3.
Vrije Universiteit Brussel
Description
Tomeetthegrowingdemandforearlyfataldiseasescreeningamonglargepopulations, current fluorescence detection instruments aiming at point-of-care diagnosis have the tendency to be low cost and high sensitivity, with a high potential for the analysis of low-volume, multiplex analytes with easy operation. In this work, we present the development of a miniaturized, high numerical aperture confocal fluorescence scanner for sub-micro-liter fluid diagnosis. It is enhanced with high-rate analyte accumulation using a pyroelectro-hydrodynamic dispensing system for generating tiny, stable sample droplets. The simplified confocal fluorescence scanner (numerical aperture 0.79, working distance 7.3 mm) uses merely off-the-shelf mass-production optical components. Experimental results show that it can achieve a high-sensitive, cost-efficient detection for sub-micro-liter, low-abundant (0.04 μL, 0.67 attomoles) fluid diagnosis, promising for point-of-care diagnosis.
Files
Miniaturized..._BOE14-6138(2023).pdf
Files
(6.7 MB)
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Additional details
Dates
- Accepted
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2023-10-01