Fig. 2 in In silico characterization of class II plant defensins from Arabidopsis thaliana

Fig. 2. Alignment between sequences obtained at the end of semi-automatic search and known class II plant defensins. Signal peptide, mature defensin and Cterminal prodomain regions are indicated by the bottom ruler. The "GXC" γ-core motif is highlighted by a magenta box; none of A. thaliana sequences have the characteristic γ-core GXCX3-9C motif between the IV and VI cysteine residues. Cysteine residues are highlighted in yellow and the lines above the sequences indicate the bond pattern between them. Cis-proline motifs are marked in grey. Predicted C-terminal prodomain recognition site is highlighted by a green box, comprising a pair of charged residues, of which the first is always a negative one. Based on this signature, we propose the RegEx "CX8-10CX2-18CX3CX2-10[GAPSIDERYW]XCX4- 17CXCX3CX2[DE][DERK]" for future studies regarding the identification of class II defensins. Charged residues of C-Terminal prodomain are highlighted in blue (positively charged) or red (negatively charged). ZmERS6, NaD1, PhD1, PhD2 presented a negatively charged C-terminal prodomain, in contrast to A7REG2 (positively charged) and A7REG4 (neutral). Sd5 is a partial sequence, and despite the known terminal residues indicated a positively charged C-terminal prodomain, its actual charge is unknown. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)