Impedance-based transport assay for SLC22A3 using HEK293 JumpIn SLC22A3 OE cells
Creators
- 1. Division of Drug Discovery and Safety, Leiden Academic Centre for Drug Research, Leiden University, the Netherlands
Description
xCELLigence is a label-free, impedance-based, real-time and non-invasive assay with which whole cell responses can be detected upon cell stimulation. Cells are seeded in wells containing gold- plated electrodes. Changes in cell properties such as proliferation, viability, morphology and adhesion can be accurately measured with xCELLigence. Read-outs of this assay can be potency values (EC50, IC50), which are calculated using either the RTCA software or GraphPad Prism. HEK293-JumpIn-OCT3 is an adherent human embryonic kidney cell line generated by RESOLUTE, which is transfected with the human Organic Cation Transporter 3 (OCT3) gene (SLC22A3) in a doxycycline-inducible expression system. This assay can be used to determine the potency (EC50) of MPP+ uptake by JumpIn-OCT3 cells. Furthermore, this assay can be used to study OCT3 inhibitors and determine their pIC50.
Files
Assays_RESOLUTE_SLC22A3.pdf
Files
(238.5 kB)
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Additional details
Related works
- Is referenced by
- Dataset: https://pubchem.ncbi.nlm.nih.gov/bioassay/1745861 (URL)