Published June 9, 2022
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Impedance-based phenotypic for SLC1A3 using HEK JumpIn SLC1A3 OE cells
Creators
- 1. Division of Drug Discovery and Safety, Leiden Academic Centre for Drug Research, Leiden University, Leiden, Netherlands
Description
Human SLC1A3 (solute carrier family 1 member 3) or EAAT1 (excitatory amino acid transporter 1), is a high affinity sodium-, proton-, and potassium-dependent glutamate transporter located in the plasma membrane.The phenotypic impedance-based assay to measure excitatory amino acid transporter (EAAT1, SLC1A3) enables detection of EAAT1 activity by uptake-induced changes of cell morphology.The mechanism behind the glutamate-induced response in dox-treated JumpIn-EAAT1 cells was attributed to initial cell swelling and subsequent spreading.
Files
Assays_RESOLUTE_SLC1A3_1.pdf
Files
(249.2 kB)
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