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Published September 14, 2021 | Version 2.1.0
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nf-core/ampliseq: Ampliseq Version 2.1.0 "Gray Steel Boa"

  • 1. QBiC
  • 2. Boehringer Ingelheim
  • 3. Linnaeus University & Stockholm University
  • 4. National Bioinformatics Infrastructure Sweden (NBIS)
  • 5. @qbicsoftware
  • 6. @SciLifeLab | Karolinska Institutet
  • 7. Science for Life Laboratory
  • 8. Seqera Labs

Description

nf-core/ampliseq version 2.1.0 "Gray Steel Boa" - 2021-09-14 Summary of changes

  • Updated documentation
  • Added export to the Swedish Biodiversity Infrastructure (SBDI) on demand (--sbdiexport)
  • Added newer versions of reference databases
  • Predict community functions on demand (--picrust) for 16S rRNA amplicons with PICRUSt2
  • Fixed some bugs
Detailed changes Added
  • #322 - Export to the Swedish Biodiversity Infrastructure (SBDI)
  • #294 - New version of the PR2 taxonomy database: 4.14.0, contains also some Bacteria, see PR2 release 4.14.0 notes
  • #294 - New version of the Unite taxonomy databases: 8.3
  • #302 - Pipeline workflow figure in README.md
  • #307 - Functional predictions with PICRUSt2, on demand with --picrust
  • #310 - Workflow figure in usage.md when using --multiple_sequencing_runs
  • #312 - Added curated GTDB 16S taxonomy: sbdi-gtdb as parameter to --dada_ref_taxonomy
  • #318 - Output information about the used reference taxonomy in a separate file in results folder dada2/ or qiime2/taxonomy
Changed
  • #313 - Relative abundance tables in qiime2/rel_abundance_tables/ on ASV level were renamed and with either DADA2 (rel-table-ASV_with-DADA2-tax.tsv) or QIIME2 classifications (rel-table-ASV_with-QIIME2-tax.tsv), if available.
Fixed
  • #306 - Sample names can now be identical to basenames of read files
  • #299, #301- Template update for nf-core/tools version 2.1
  • #303 - Reverse primer of PacBio and IonTorrent reads should be now given to --RV_primer in usual direction (before: reverse complement)
  • #305 - --max_len now accepts integers as expected
  • #314, #315 - ASV fasta input via --input fixed (was broken in 2.0.0) and a test profile was added.
Dependencies
  • #299 - Updated MultiQC to v1.10
  • #319 - Updated DADA2 from 1.18.0 to 1.20.0

Files

nf-core/ampliseq-2.1.0.zip

Files (3.4 MB)

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