Compositional and Quantitative Insights Into Bacterial and Archaeal Communities of South Pacific Deep-Sea Sponges (Demospongiae and Hexactinellida)

ACKNOWLEDGMENTS This work is dedicated to Hans Tore Rapp, coordinator of the H2020-SponGES project, mentor and friend. We thank Andrea Hethke, Ina Clefsen, and the CRC1182 Z3 team (Katja Cloppenborg-Schmidt, Malte Rühlemann, John Baines) for assistance with the amplicon pipeline. We greatly acknowledge the crew and scientific party of RV Sonne cruise SO254, as well as the ROV Kiel 6000 team for their valuable support at sea. We also thank Sven Rohde, Tessa Clemens and the entire benthic invertebrate team of the RV Sonne Cruise SO254 for their assistance in sample collection, processing and cataloging. We thank Henry Reiswig for advice on identification of hexactinellid samples. Sample collection was carried out under the "Application for consent to conduct marine scientific research in areas under national jurisdiction of New Zealand (dated 7.6.2016)." This is publication 68 of Senckenberg am Meer Metabarcoding and Molecular Laboratory. We also thank the reviewers for their comments, which helped to improve this manuscript. DATA AVAILABILITY STATEMENT Sequences were deposited at NCBI as BioProjects with accession IDs PRJNA552490 and PRJNA552540. AUTHOR CONTRIBUTIONS PS, UH, KaB, and GS designed the experiments. GS, KaB, KrB, GW, PS, SK, and PA performed the experiments. GS, DE, KaB, KrB, UH, and PS analyzed the data. GS, KaB, KrB, PS, and UH wrote the manuscript. GS, GW, KaB, KrB, MD, SM, UH, and PS reviewed and edited the manuscript. MD identified hexactinellid specimens. MK identified demosponge specimens. SM provided initial identification and curation of taxonomic vouchers of sponges. FUNDING This study was funded by the European Union's Horizon 2020 Research and Innovation Program to UH under Grant Agreement No. 679849 ('SponGES') and by the DFG Collaborative Research Center CRC1182-TP B1 ("Metaorganisms") to UH. PS acknowledges funding by the Federal Ministry of Education and Research (BMBF) for the cruise SO254, grant 03G0254A, PORIBACNEWZ. GW acknowledges funding by LMU Munich's Institutional Strategy LMU excellent within the framework of the German Excellence Initiative. SUPPLEMENTARYMATERIAL The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb. 2020.00716/full#supplementary- material