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Published January 27, 2015 | Version v1
Poster Open

Glass: A Multi-Platform Specimen Supporting Substrate for Precision Single Molecule Studies of Membrane Proteins

Creators

  • 1. Johns Hopkins University
  • 2. Cal Poly Pomona
  • 3. Weill Cornell Medicine
  • 4. University of Missouri
  • 5. Stanford University

Description

1) Method for for integration of high resolution biological AFM with other powerful state of the art optical techniques

2) Straight-forward cleaning procedure for treatment of glass for Microscopy and Micromachining applications

3) Molecular high resolution imaging of bacteriorhodopsin and Sec translocase on glass supports

4) Direct observation of protein-protein interactions

5) Atomic Force Microscopy measurements of surface roughness of Glass, Mica, Lipid and comparison of several glass chemical treatments for Microscopy

1. READ THE PEER-REVIEWED PUBLICATION HERE

2. READ THE PEER-REVIEWED PUBLICATION HERE

Files

Biophysical Society Meeting 2015 Poster and Abstract.pdf

Files (2.8 MB)

Additional details

References

  • Chada, N., Sigdel, K., Gari, R. et al. Glass is a Viable Substrate for Precision Force Microscopy of Membrane Proteins. Sci Rep 5, 12550 (2015). https://doi.org/10.1038/srep12550
  • Chada, N., Sigdel, K., Matin, T., Sanganna Gari, R.R., Mao, C., Randall, L. and King, G., 2013. Glass is a viable substrate for atomic force microscopy of membrane proteins. Bulletin of the American Physical Society, 58. http://meetings.aps.org/Meeting/PSF13/Event/206952
  • Chada , Nagaraju . Growth and Characterization of Transition Metal Oxides for Chemical Sensor Applications: Setting up Initiated Hot Wire Chemical Vapor Deposition System. (2010).
  • Chada, N., Sigdel, K.P., Matin, T.R., Gari, R.R.S., Mao, C., Randall, L.L. and King, G.M., 2014. Glass is a viable substrate for atomic force microscopy of membrane proteins. Biophysical Journal, 106(2), p.458a., https://www.cell.com/biophysj/pdf/S0006-3495(13)03853-8.pdf
  • Chada, Nagaraju. (2017, December 23). Watching Biological Nanomotors At Work: Insights From Single-Molecule Studies. Zenodo. https://doi.org/10.5281/zenodo.3996607
  • Marsh, B.P., Chada, N., Sanganna Gari, R.R. et al. The Hessian Blob Algorithm: Precise Particle Detection in Atomic Force Microscopy Imagery. Sci Rep 8, 978 (2018). https://doi.org/10.1038/s41598-018-19379-x
  • N. Chada, K. Chattrakun, B. P. Marsh, C. Mao, P. Bariya, G. M. King, Single-molecule observation of nucleotide induced conformational changes in basal SecA-ATP hydrolysis. Sci. Adv. 4, eaat8797 (2018).
  • Chada, Nagaraju et al. Single Molecule Studies of Membrane Proteins on Glass Substrates using Atomic Force Microscopy. (Zenodo, 2014). doi:10.5281/zenodo.4016547