Features of the condition of the neuroendocrine-immune complex in different constellations of entropies of morpho-functional immune subsystems in rats

Popadynets’ Oleksandr O., Bilas Volodymyra R., Flyunt Igor-Severyn S., Badiuk Nataliya S., Zukow Walery, Gozhenko Anatoliy I., Popovych Igor L. Features of the condition of the neuroendocrine-immune complex in different constellations of entropies of morpho-functional immune subsystems in rats. Journal of Education, Health and Sport. 2020;10(8):214-229. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2020.10.08.025

https://apcz.umk.pl/czasopisma/index.php/JEHS/article/view/JEHS.2020.10.08.025

https://zenodo.org/record/3987208

The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019.

© The Authors 2020;

This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland

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Received: 20.07.2020. Revised: 25.07.2020. Accepted: 16.08.2020.

FEATURES OF THE CONDITION OF THE NEUROENDOCRINE-IMMUNE COMPLEX IN DIFFERENT CONSTELLATIONS OF ENTROPIES OF MORPHO-FUNCTIONAL IMMUNE SUBSYSTEMS IN RATS

Oleksandr O. Popadynets’¹, Volodymyra R. Bilas^1,2, Igor-Severyn S. Flyunt³,

Nataliya S. Badiuk¹, Walery Zukow⁴, Anatoliy I. Gozhenko¹, Igor L. Popovych²

¹State Enterprise Ukrainian Research Institute for Medicine of Transport, Odesa, Ukraine bilas1940@gmail.com; prof.gozhenko@gmail.com

²OO Bohomolets’ Institute of Physiology, Kyїv, Ukraine i.popovych@biph.kiev.ua

³Ivan Franko State Pedagogical University, Drohobych, Ukraine igorf3007@ukr.net

⁴Nicolaus Copernicus University, Torun, Poland w.zukow@wp.pl

Abstract

Background. We have previously shown that the Entropy (E) of the Thymocytogram (T), Splenocytogram (S) as well as Leukocytogram (L) and Immunocytogram (I) of the blood in rats are virtually independent of each other. The purpose of this study was to clarify the characteristics of the condition of the neuroendocrine-immune complex in different constellations of E(s) of these four morpho-functional immune subsystems. Materials and methods. Experiment was performed on 108 healthy Wistar rats (48 male and 60 female) weighing 240-290 g divided into 8 groups. Animals of the first group remained intact. Instead, the other rats received various balneofactors for 6 days. The day after the completion of the drinking/application course the parameters of neuroendocrine-immune complex were registered. Results. The method of cluster analysis created four homogeneous groups of rats, significantly different from each other in the constellation of E of four morpho-functional immune subsystems. The members of the first cluster (n=31) are characterized by elevated levels of E (Z±SE) of I (1,12±0,16), T (1,00±0,14) and L (0,88±0,12) at the normal E level of S (0,14±0,16). The members of the second cluster (n=21) are characterized by reduced E level of L (-1,59±0,16) in combination with increased levels of I (0,80±0,17) and T (0,81±0,15) at the normal E level of S (0,13±0,16). A characteristic feature of the members of the third cluster (n=28) is reduced E of T (-1,01±0,17) at normal E levels of S (-0,33±0,23), I (-0,19± 0,14 ) and L (0,35±0,20). The last cluster (n=28) is characterized by reduced E of I (-1,36± 0,14), while the E of other immune subsystems are at the borderline levels: L (-0,52±0,16), S (0,49±0,15), T (0,60±0,15). The method of discriminant analysis revealed 15 parameters of the neuroendocrine-immune complex, the set of which four clusters of Entropies differ from each other with an accuracy of 95%. Conclusion. Each constellation of independent Entropies of the thymocytogram, splenocytogram, immunocytogram and leukocytogram is accompanied by a specific constellation of parameters of the neuroendocrine-immune complex which indicates their natural functional relationships.

Key words: Entropy of thymocytogram; splenocytogram; immunocytogram and leukocytogram; autonomic nervous; endocrine and immune parameters; female and male rats.