MiRNA expression profile in serum of osteoarthritis patients

Dariusz Szala, Joanna Czech, Marzena Skrzypa, Marta Kopanska, Dorota Hanf-Osetek, Krzysztof Gargasz, Grzegorz Guzik, Slawomir Snela, Izabela Zawlik. MiRNA expression profile in serum of osteoarthritis patients. Journal of Education, Health and Sport. 2019;9(11):11-30. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2019.09.11.001

https://apcz.umk.pl/czasopisma/index.php/JEHS/article/view/JEHS.2019.09.11.001

https://zenodo.org/record/3528173

 

 

 

 

 

The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019.

© The Authors 2019;

This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland

Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike.

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The authors declare that there is no conflict of interests regarding the publication of this paper.

 

Received: 25.10.2019. Revised: 04.11.2019. Accepted: 04.11.2019.

 

 

 

 

 

MiRNA expression profile in serum of osteoarthritis patients

 

 

Dariusz Szala 3*, Joanna Czech 1,2*, Marzena Skrzypa 1,2*, Marta Kopanska 1,2, Dorota Hanf-Osetek 3, Krzysztof Gargasz 4, Grzegorz Guzik 6, Slawomir Snela 3,5, Izabela Zawlik 1,2**

 

 

¹Laboratory of Molecular Biology, Centre for Innovative Research in Medical and Natural Sciences, Faculty of Medicine, University of Rzeszow, Poland;

²Department of Genetics, Chair of Molecular Medicine, Faculty of Medicine, University of Rzeszow, Poland;

³Department of Orthopaedic and Traumatology, University Hospital No 2, Rzeszow, Poland;

⁴Data Analysis Laboratory, Centre for Innovative Research in Medical and Natural Sciences, Faculty of Medicine, University of Rzeszow, Poland;

⁵Institute of Physiotherapy, Faculty of Medicine, University of Rzeszow, Poland

⁶Department of Oncological Orthopaedics, Specialist Hospital in Brzozów – Podkarpacie Oncological Centre, Poland

 

 

 

 

^*Equal contribution

 

^**Corresponding author: tel. +48 17 851 68 10, izazawlik@yahoo.com 

Laboratory of Molecular Biology, Center for Innovative Research in Medical and Natural Sciences, Faculty of Medicine, University of Rzeszow, Warzywna 1A, 35-310 Rzeszow, Poland

 

 

 

 

 

Abstract

Introduction: Osteoarthritis (OA) is the most common form of arthritis that affects millions of people worldwide. Despite advances in medicine, OA still remains incurable. Scientists are still seeking candidates for biomarker-based risk stratification and the early detection of disease. Measurement of miRNAs in the serum may become a powerful tool in the development of diagnostic biomarker.
Purpose: The aim of this study was to examine the expression profiles of 20 miRNAs in the serum of patients with OA.

Methods: We used RT-qPCR method to measure the expression profile of selected miRNAs.

Results: Six miRNAs showed differential expression between OA and normal serum samples. miR-146a-5p and miR-98-5p were significantly upregulated (P < 0.05), while miR-222-3p, miR-22-3p, miR-27a-3p and miR-93-5p were downregulated (P < 0.05) compared to the control group. To the best of our knowledge, this is the first work that shows that expression of these six miRNAs is different between serum OA samples and healthy controls. However, we did not find any statistically significant relationship between the miRNAs expression and clinical characteristics.

Conclusions: Our findings suggest that miRNAs can be used as potential biomarkers for OA, but further research is needed to assess the usefulness of serum miRNAs in miRNA-based prognostic and therapeutic approaches.

 

Key words: expression; miRNA; osteoarthritis; serum