World Academy of Science, Engineering and Technology
Published September 3, 2014 | Version 9999401
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Growth Behaviors, Thermostable Direct Hemolysin Secretion and Fatty Acid Profiles of Acid-adapted and Non-adapted Vibrio parahaemolyticus

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Three strains of Vibrio parahaemolyticus (690, BCRC
13023 and BCRC 13025) implicated in food poisoning outbreaks in
Taiwan were subjected to acid adaptation at pH 5.5 for 90 min. The
growth behaviors of acid-adapted and non-adapted V.
parahaemolyticus in the media supplemented with various nitrogen
and carbon sources were investigated. The effects of acid adaptation
on the thermostable direct hemolysin (TDH) secretion and fatty acid
profiles of V. parahaemolyticus were also examined. Results showed
that acid-adapted and non-adapted V. parahaemolyticus 690, BCRC
13023 and BCRC 13025 grew similarly in TSB-3% NaCl and basal
media supplemented with various carbon and nitrogen sources during
incubation period. Higher TDH secretion was noted with V.
parahaemolyticus 690 among the three strains. However, acid-adapted
strains produced less amounts of TDH than non-adapted strains when
they were grown in TSB-3% NaCl. Additionally, acid adaptation
increased the ratio of SFA/USFA in cells of V. parahaemolyticus
strains.

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References

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