Multi-colour SMLM images of untreated and drug-treated Escherichia coli (LB, exponential phase)

This dataset and CARE model is part of the publication "Transertion and cell geometry organize the Escherichia coli nucleoid during rapid growth".

It contains all SMLM images that were used for the publication, as well as the single-cell regions of interest for analyses.

Cells were grown to exponential phase in LB Lennox and antibiotics were added for 0-60 min. Cultures were then chemically fixed, permeabilised and imaged for the nucleoid (JF₆₄₆-Hoechst) and membranes (Nile Red) using PAINT. The strain (NO34) expresses a MreB^sw-sfGFP fusion protein from the native chromosomal locus. It was a kind gift from Zemer Gitai (Ouzounov et al., 2016).

More information can be found in the publication.