Data for: Simultaneous subset tracing and miRNA profiling of tumor-derived exosomes via dual-surface-protein orthogonal barcoding
Creators
- 1. Shanghai Jiao Tong University
- 2. Southwest University
- 3. Xiamen University
Description
The clinical potential of miRNA-based liquid biopsy has been largely limited by the heterogeneous sources in plasma and tedious assay processes. Here we develop a precise and robust one-pot assay called dual-surface-protein-guided orthogonal recognition of tumor-derived exosomes and in-situ profiling of microRNAs (SORTER) to detect tumor-derived exosomal miRNAs and enhance the diagnostic accuracy of prostate cancer (PCa). The SORTER utilizes two allosteric aptamers against exosomal marker CD63 and tumor marker EpCAM to create an orthogonal labeling barcode and achieve selective sorting of tumor-specific exosome subtypes. Furthermore, the labeled barcode on tumor-derived exosomes initiated targeted membrane fusion with liposome probes to import miRNA detection reagents, enabling in-situ sensitive profiling of tumor-derived exosomal miRNAs. With a signature of six miRNAs, SORTER differentiated PCa and benign prostatic hyperplasia with an accuracy of 100%. Notably, the diagnostic accuracy reached 90.6% in the classification of metastatic and non-metastatic PCa. We envision that the SORTER will promote the clinical adaptability of miRNA-based liquid biopsy.
Notes
All statistical analyses were performed at 95% (P < 0.05) CIs using OriginPro 2018, GraphPad Prism (v.8.0), and R software (version 4.1.2).
Funding provided by: National Key R&D Program of China
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100012166
Award Number: 2022YFA1305200
Funding provided by: National Natural Science Foundation of China
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100001809
Award Number: 22004084
Funding provided by: Program for Changjiang Scholars and Innovative Research Team in University
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100018621
Award Number: Grant IRT13036
Funding provided by: National Key Research and Development Program of China
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100012166
Award Number: 2019YFA0905800
Funding provided by: National Natural Science Foundation of China
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100001809
Award Number: 21927806
Funding provided by: National Natural Science Foundation of China
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100001809
Award Number: 21735004
Funding provided by: National Natural Science Foundation of China
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100001809
Award Number: 82072847
Funding provided by: National Natural Science Foundation of China
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100001809
Award Number: 22022408
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Fig.3B-Fluorescence_spectra_analysis.csv
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Additional details
Related works
- Is cited by
- 10.21203/rs.3.rs-2404819/v1 (DOI)