Isolation and Identification of Endophytic Fungi from Ocimum sanctum

Endophytic fungi are a diverse group of microorganisms that inhabit the internal tissues of plants without causing any visible harm. These fungi have gained significant attention due to their potential for producing bioactive compounds with various applications in medicine, agriculture, and industry. In this study, we aimed to isolate and identify endophytic fungi from the Ocimum sanctum plant, commonly known as holy basil. The methods employed for isolation and identification included surface sterilization, tissue maceration, fungal isolation, morphological and microscopic characterization. A total of five endophytic fungal strains were isolated and identified. The results revealed a high diversity of endophytic fungi associated with Ocimum sanctum. Furthermore, several potential bioactive compounds were detected in the isolated fungi, further emphasizing their importance. This study contributes to the knowledge of endophytic fungi associated with Ocimum sanctum and provides a foundation for further investigation into their biotechnological potential.