Published December 14, 2020 | Version v1
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Video data describing bioluminescent detection of isothermal DNA amplification in microfluidic generated droplets and artificial cells

Description

Video data of loop-mediated isothermal nucleic acid amplification (LAMP) and bioluminescent assay in real time (BART) in microfluidicaly produced droplets. The videos are the data used to generate still images for figures in the source publication "Bioluminescent detection of isothermal DNA amplification in microfluidic generated droplets and artificial cells".

Details of the videos acquisition parameters are as follows:

  • Video 1: LAMP-BART droplets in tubing - no DNA template control (Figure 2a) (assay duration 600s, frames 1 per 10s, 60 frames, final
  • Video 2 frames per second) Video 2: LAMP-BART droplets in tubing - positive DNA template control (Figure 2b) (assay duration 1800s, frames 1 per 10s, 180 frames, final video 10 frames per second)
  • Video 3: LAMP-BART parked droplets (Figure 3) (assay duration 600s, frames 1 per 10s, 60 frames, final video 3.3 frames per second)
  • Video 4: LAMP-BART DNA gradient in tubing (Figure 4) (assay duration 1800s, frames 1 per 10s, 180 frames, final video 10 frames per second)
  • Video 5: LAMP-BART eDIBs (Figure 5) (assay duration 1570s, frames 1 per 10s, 157 frames, final video 5.2 frames per second)
  • Video 6: One to four core LAMP-BART eDIBs (Figure 6) (assay duration 1200s, frames 1 per 10s, 120 frames, final video 5 frames per second)

When referencing please use:

Hardinge, P., Baxani, D. K., McCloy, T., Murray, J. A. H. & Castell, O. K. Bioluminescent detection of isothermal DNA amplification in microfluidic generated droplets and artificial cells. Sci Rep 10, 21886 (2020).

Notes

The work was supported by funding from: H2020-EU.1.2.2.—FET Proactive Grant Agreement ID: 824060 "ACDC". Wellcome Trust (ISSF 204824-Z-16-Z)

Files

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Additional details

Related works

Is derived from
Journal article: 10.1038/s41598-020-78996-7 (DOI)

Funding

ACDC – Artificial Cells with Distributed Cores to Decipher Protein Function 824060
European Commission