Complete Z-depth imaging for GFP and JPH2 fluorescence and WGA staining in a JPH2+ ARVM at day 4 in culture.

Supplementary Figure 1. Video shows a complete view across the Z-axis for GFP (left) and JPH2 (middle) fluorescence and WGA (right) staining in a JPH2+ ARVM at day 4 of culture. The top cell has greater GFP and JPH2 fluorescence but internal WGA staining is little different from the bottom cell. JPH2 staining intensity is much more punctate in the top cell. Z-depth was 1 µm.