Published July 31, 2021 | Version v1
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Fig. 4 in Metabolite profiling reveals a role for intercellular dihydrocamalexic acid in the response of mature Arabidopsis thaliana to Pseudomonas syringae

  • 1. * & Vineland Research and Innovation Centre, 4890 Victoria Ave North Box 4000, Vineland Station, Ontario, L0R 2E0, Canada & * & McMaster University, 1280 Main St W, Hamilton, Ontario, L8S 4L8, Canada & * & Brock University, 1812 Sir Isaac Brock Way, St Catharines, Ontario, L2S 3A1, Canada

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Fig. 4. Quantification of dihydrocamalexic acid (DHCA) (m/z 247.0541, [C12H10N2O2S þ H]) in intercellular washing fluids (IWFs). DHCA levels measured in IWFs from Col-0, cyp71a12/cyp71a13, and cyp71b15 (all 7-weeks post-germination) 24 h after inoculation with P. syringae (Pst) or 10 mM MgCl2 (mock-inoculation) measured by UPLC-MS electrospray ionization in positive mode (ESI+). Values represent the mean ± standard deviation of three sample replicates (n = 3). Different letters indicate statistically significant differences (one-way ANOVA, Tukey's honestly significant difference [HSD], P <0.05). Standard curves were prepared using synthetic DHCA (2 pg–6 μg on column).

Notes

Published as part of Kempthorne, Christine J., Nielsen, Alexander J., Wilson, Daniel C., McNulty, James, Cameron, Robin K. & Liscombe, David K., 2021, Metabolite profiling reveals a role for intercellular dihydrocamalexic acid in the response of mature Arabidopsis thaliana to Pseudomonas syringae, pp. 1-13 in Phytochemistry (112747) 187 on page 6, DOI: 10.1016/j.phytochem.2021.112747, http://zenodo.org/record/8259052

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Journal article: 10.1016/j.phytochem.2021.112747 (DOI)
Journal article: urn:lsid:plazi.org:pub:FF8F325EFF9CFA20180A8826FFD0105E (LSID)
Journal article: https://zenodo.org/record/8259052 (URL)