Published August 17, 2023 | Version V1.0
Technical note Open

Induction of Dopaminergic Neuronal Progenitors From iPSCs Using Microfabricated Disk

Creators

  • 1. McGill University

Description

This protocol describes how to generate dopaminergic neural progenitor cells (DPCs) from induced-pluripotent stem cells (iPSCs) using microfabricated embryoid body disk device (EB disk).

This protocol describes how to:

  • Thaw and culture dopaminergic neural progenitor cells (DPCs).
  • Plate DPCs in a 96 well format to be differentiated into dopaminergic neurons (DNs)

Files

EDDU-018-01_Dopaminergic Neurons Induction using EB-disk_v1.0.pdf

Files (4.1 MB)