Reduce the Risk of Oxidation and Pathogenic Bacteria Activity by Moringa oleifera Different Leaf Extract Grown in Sudan

High Performance Liquid Chromatography (HPLC) used in this study to identified Polyphenol constituents of Moringa oleifera leaf extract by different methods (aqueous, ethanol, ethyl acetate and chloroform), it contain gallic acid, Chlorogenic acid, Catechin, Coffeic acid, Rutin, Pyro catechol, Coumaric acid, Vanillin, Ferulic acid1, Naringenin, Propyl Gallate, 4`,7-Dihydroxyisoflavone, and Cinnamic Acid at conc. (µg/15 mg) in all extracts. Ellagic acid gave the highest concentration when extracted by ethyl acetate Caffeine gave the lowest concentration. in all different extract, The effect of moringa (aqueous, ethanol, ethyl acetate and chloroform) leaf extracts against four different pathogenic bacteria Salmonella typhimurium, Pseudomonas aeruginosa, Escherichia coli, and Bacillus cereus, were examined using Mueller Hinton Agar and measuring inhibition zone (diameter mm), were found that, there were a significant different of all moringa leaf extracts against bacteria. The study was conducted to determine the polyphenol constituents of Moringa oleifera aqueous, ethanol, ethyl acetate and chloroform leaf extract. The effect of Moringa oleifera (aqueous, ethanol, ethyl acetate and chloroform) leaf extracts against four different pathogenic bacteria.