ImageSURF MOAB2 Image Example
Creators
- 1. Wicking Dementia Research and Education Centre, Faculty of Health, University of Tasmania, Tasmania 7000, Australia
- 2. Wicking Dementia Research and Education Centre, Faculty of Health, University of Tasmania, Tasmania 7000, Australia; School of Medicine, Faculty of Health, University of Tasmania, Tasmania 7000, Australia
Description
A set of 2000x2000 raw confocal fluorescence images of MOAB2-labelled cortex from APPswe/PS1dE9 mouse models of Alzheimer's disease, un-annotated intensity scaled 8-bit images, sparsely annotated pixel labels and reference segmentation examples. Pixels are annotated as signal (red 0xFFFF0000) and background (blue 0xFF0000FF).
Images were captured as stitched 12-bit greyscale single-plane images and cropped to size. Image acquisition was performed at 561nm excitation and 615nm emission wavelengths using a Perkin Elmer Ultraview VOX imaging system with Nikon Eclipse Ti inverted microscope, 20x objective lens, x-y motorised stage, Hamamatsu ORCA-R2 C10600-10B CCD camera and Volocity 6.3 software package.
These images are shared as examples for the ImageSURF segmentation plugin for ImageJ2/FIJI (https://doi.org/10.5281/zenodo.818109).
Files
imagesurf-moab2-example.zip
Files
(156.3 MB)
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