DEVELOPMENT AND VALIDATION OF STABILITY INDICATING RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF SITAGLIPTIN AND ERTUGLIFLOZIN IN PHARMACEUTICAL DOSAGE FORMS

Another technique was laid out for concurrent assessment of Ertugliflozin and sitagliptin by RP-HPLC strategy. Chromatogram was gone through Inertsil ODS C185m (4.6 x 250mm). Portable stage containing Phosphate support and Acetonitril in the proportion of 30:70 was siphoned through section at a stream pace of 1ml/min. Support utilized at pH 4.6. Temperature was kept up with at Encompassing. Streamlined frequency for Sitagliptin and Ertugliflozin was 235 nm. Maintenance season of Sitagliptin and Ertugliflozin were viewed as 2.395min and 3.906min. The % virtue of Sitagliptin and Ertugliflozin was viewed as 100.6% and 101.3% individually. The framework appropriateness boundaries for Sitagliptin and Ertugliflozin, for example, hypothetical plates and following variable were viewed as 1.3, 1012.4and 1.2, 1848.2 the goal was viewed as 9.0.The linearity study for Sitagliptin and Ertugliflozin was found in fixation scope of 1μg-5μg and 100μg-500μg and relationship coefficient (r2) was viewed as 0.999 and 0.999, % mean recuperation was viewed as 100.1% and 100.4%, %RSD for repeatability was0.31 and 0.38, % RSD for halfway accuracy was 0.12 and 0.15 separately. The accuracy study was exact, powerful and repeatable. LOD esteem was 2.94 and 3.03, and LOQ esteem was 9.87 and 10.1 separately The consequences of study showed that the proposed RP‐HPLC technique is a basic, exact, exact, rough, vigorous, quick and reproducible, which might be valuable for the normal assessment of Ertugliflozin and sitagliptin in drug measurements structure.

Keywords: Ertugliflozin, sitagliptin, RP‐HPLC, Simultaneous estimation.