Published July 17, 2023
| Version v0
Dataset
Open
Structural and Molecular Analysis of Adult Mouse Astrocytes and Vascular Connectivity in the Cortex and Hippocampus
Creators
- 1. EPFL, Blue Brain Project
- 2. EPFL, Bioimaging and Optics Platform
- 3. EPFL, Bioinformatics Competence Center
- 4. Université de Lausanne, Bioinformatics Competence Center
Description
After image acquisition (0-RAW_CL230331_E2_serie1) and deconvolution (1-Deconvolved_CL230331_E2_serie1) using confocal microscopy and the SVI Huygens software,respectively, the image processing was conducted using Imaris, Fiji, and Matlab software. This process involved a sequence of manual operations (2-Imaris_surfaces_CL230331_E2_serie1) and custom Groovy scripts (5-Groovy scripts).
The dataset analysis (3-Imaris_final_CL230331_E2_serie1_ims) allowed for a deeper investigation of morphological and molecular properties of adult mouse astrocytes (4-Image analysis_CL230331_E2_serie1) in two brain regions, the Isocortex and the Hippocampus, known to be interconnected to support multiple cognitive functions.
Files
0-0 Overall workflow.png
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(20.9 GB)
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Additional details
Related works
- Is published in
- Publication: 10.1002/glia.24594 (DOI)