ESTABLISH OF PROTOCOL FOR CUCUMIS MELO L. USING DIFFERENT HORMONES THROUGH PLANT TISSUE CULTURE TECHNIQUE

Abstract

Melon (Cucumis melo L.) is economically importance plants in agricultural production in the province of Balochistan, Pakistan. In this study, we developed various regeneration and transformation protocols and we examined the different earlier morphological characters of Cucumis melo on MS medium with different combinations and concentration of plant growth regulators (PGRs) (Auxin, cytokinin, and gibberellin). Result showed that the maximum value of callus was obtained when 0.50mgl^-1 of BAP was combined with 0.50mgl^-1 of 2,4-D about 52%. The maximum regeneration percentage was obtained by the combination of 1mgl^-1 NAA, 0.5 mgl^-1 BAP and 0 mgl^-1 KIN about 80%. Although, the mean number of shoots were displayed a maximum value in MS medium having the combination of 2.5mgl^-1 NAA, 1mgl^-1 BAP, and 0mgl^-1 KIN about 2.4± 0.3. In addition, when the treatment was treated with 1.5mgl^-1 BAP, and 0.5mgl^-1 IBA showed a significant response on morphological parameters of studied plants. Similarly, the maximum number of roots, root and shoot length was observed when treated with 2 mgl^-1 of IAA and IBA respectively. We assume that statement of the research findings presented here lead to for further studies on in vitro propagation of melon plants.