Development of oxidative stress in rats of different sexes subjected to immobilization stress of different durations

Denefil, O.V. and Pokryshko, A.O. Development of oxidative stress in rats of different sexes subjected to immobilization stress of different durations. Journal of Education, Health and Sport. 2023;15(1):77-89. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2023.15.01.010

https://apcz.umk.pl/JEHS/article/view/42115

https://zenodo.org/record/8125014

The journal has had 40 points in Ministry of Education and Science of Poland parametric evaluation. Annex to the announcement of the Minister of Education and Science of December 21, 2021. No. 32343.

Has a Journal's Unique Identifier: 201159. Scientific disciplines assigned: Physical Culture Sciences (Field of Medical sciences and health sciences); Health Sciences (Field of Medical Sciences and Health Sciences).

Punkty Ministerialne z 2019 - aktualny rok 40 punktów. Załącznik do komunikatu Ministra Edukacji i Nauki z dnia 21 grudnia 2021 r. Lp. 32343. Posiada Unikatowy Identyfikator Czasopisma: 201159.

Przypisane dyscypliny naukowe: Nauki o kulturze fizycznej (Dziedzina nauk medycznych i nauk o zdrowiu); Nauki o zdrowiu (Dziedzina nauk medycznych i nauk o zdrowiu).

© The Authors 2023;

This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland

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Received: 16.05.2023. Revised: 24.05.2023. Accepted: 25.06.2023. Published: 07.07.2023.

DEVELOPMENT OF OXIDATIVE STRESS IN RATS OF DIFFERENT SEXES SUBJECTED TO IMMOBILIZATION STRESS OF DIFFERENT DURATIONS

O.V. Denefil, A.O. Pokryshko

I. Ya. Horbachevsky Ternopil National Medical University

Denefil Olha Volodymyrivna – Doctor of Science in Medicine, Professor, head of the Department of Pathological Physiology at I. Ya. Horbachevsky Ternopil National Medical University Ministry of Health of Ukraine

Pokryshko Alina Olehivna – Assistant of the Department of Disaster and Military Medicine at I. Ya. Horbachevsky Ternopil National Medical University Ministry of Health of Ukraine

Abstract

Introduction. The problem of stress is currently one of the main problems not only in Ukraine, but also in the whole world. True, stress does not lead to damage in everyone, some adapt to stress. Studying the impact of different durations of stress, in particular, clarifying the mechanisms of their impact on the body, is relevant.

The purpose of the study is to evaluate the processes of lipid peroxidation in the blood of rats of different sexes that have experienced stress of different durations.

Materials and methods. Experiments were performed on 56 white male Wistar rats aged 3.5-4 months. The animals were divided into 4 groups: 1 – control, 2 – stress during 1 hour (Stress 1), 3 – stress during 2 hours (Stress 2), 4 – stress during 3 hours (Stress 3). Stress was induced by gently fixing the animal's on a plate with its back down. Superoxide dismutase and catalase activity (SOD, CAT), the concentration of diene and triene conjugates (DC, TC), Schiff's bases (SB), TBA-active products (TBA-ap) were determined in blood serum.

Research results and their discussion. In male rats with Stress 1, compared to the control, there was an increase of DC by 11.1%, TC – by 14.9%, SB – by 26.3%, TBA-ap – by 27.5%, SOD – by 12.5%, CAT – by 31.3%. In female rats with Stress 1, compared to the control, there was an increase in DC by 4.2%, TC – by 8.9%, SB – by 45.2%, TBA-ap – by 14.1%, SOD – by 15.5%, and CAT – by 7.3%. Such results indicated greater activation of lipid peroxidation processes in males and greater activation of antioxidants in females.

In males with Stress 2, compared to the control, the content of DC increased by 83.1%, TC – by 97.4%, SB – by 65.1%, TBA-ap – by 2.6 times, SOD decreased by 9.5%, CAT increased by 44.5%. In females with Stress 2 the content of DC increased by 30.3%, TC – by 28.2%, SB – by 35.5%, TBA-ap – by 85.6%. SOD was at the same level as in the control, CAT increased by 23.1%. Such results indicated a greater activation of lipid peroxidation processes in males and a decrease in their antioxidant activity.

In males with Stress 3, compared to the control, the content of DC increased by 2.6 times, TC – by 2.8 times, SB – by 75.8%, TBA-ap – by 3.2 times. SOD decreased sharply by 41.9%, and CAT increased by 66.6%. In females with Stress 3 the content of DC increased by 2.5 times, TC – by 2.5 times, SB – by 45.2%, TBC-ap – by 2.9 times. SOD sharply decreased by 19.6%, and CAT increased by 35.4%. Such results indicated a significant activation of lipid peroxidation processes when antioxidant activity was depleted in males.

It was also noted the presence of erosions on the mucous membrane precisely during stress lasting 3 hours, when spot hemorrhages appeared after 2 hours, and hyperemia of the gastric mucosa - after 1 hour.

Conclusion. Animals adapt to stress lasting 1 hour. With stress that lasts for 2 hours, disadaptation of animals occurs. With stress that lasts for 3 hours, the animal's body becomes exhausted. In male rats, lipid peroxidation processes occur at a higher level, compared to females.

KEY WORDS: lipid peroxidation, antioxidant activity, stress, blood, rats.