A simple, rapid and highly sensitive magnetic beads ELISA for detection of SARS CoV-2 antibodies (IgG) in human plasma samples as point of the care assay
Description
Aim: Pandemic outbreak of coronavirus (SARS CoV-2) is going on for last 3 years. The people are vaccinated with different vaccines targeting S protein. Therefore, it is essential to have an assay, which can detect the different parts of virus as serological assay and can be performed as point of the care test, hence in this work, we decided to develop such assay with the help of magnetic beads.
Methods: The magnetic beads ELISA was developed in a microtube. The viral ligand specific magnetic beads were used to detect the nucleoprotein (NP) specific IgG antibodies in human plasma samples. The results were read with naked eyes as well as with professional ELISA readers.
Results: 7 µl magnetic beads were suitable to detect the presence of NP specific antibodies. The assay needs only magnetic rack and pipettor to be performed. The results were available within 30 minutes. The positive results were observed as yellow color visually, but also read in ELISA reader as OD values. The sensitivity of this assay was 1:108 dilutions. The cross-reaction panel was negative with different pathogens and negative human plasma.
Conclusion: In this work, it should be the first report in literature about the development of a magnetic beads ELISA as point of the care assay, which is reproducible, highly sensitive, robust and easy to perform. It was used to detect the presence of NP specific IgG antibodies in the plasma samples successfully. This assay can be used as professional assay, where the results can be measured with ELISA reader. This assay may be suitable in small clinics also under field conditions. It can be used to detect the SARS CoV-2 infection in vaccinated persons (S protein-based vaccines) along with non-vaccinated population in latent and active phase.
Files
magnetic beads ELISA SARS CoV-2_preprint.pdf
Files
(465.3 kB)
| Name | Size | Download all |
|---|---|---|
|
md5:f9689415e50c1930afc3a55328f5ead9
|
465.3 kB | Preview Download |
Additional details
References
- Bhatia, S. (2021). A simple method for isolation of rest of trypsinized stem cells with magnetic beads. Jurnal Teknologi Laboratorium, 10(1), 01–02.
- Bhatia, S. (2022). A simple and quick isolation method to obtain a huge number of fibroblasts like mesenchymal stem cells from human umbilical cord. Jurnal Teknologi Laboratorium, 11(2), 60–64. https://doi.org/10.29238/teknolabjournal.v11i2.385
- Bhatia S. Pitfalls found in SARS CoV-2 specific test performance during the comparison between WHO recommended method and a commercial test. Atlantic J Med Sci Res. 2023;3(1):22-6. DOI: 10.5455/atjmed.2022.12.024
- Huang, A.T., Garcia-Carreras, B., Hitchings, M.D.T. et al. A systematic review of antibody mediated immunity to coronaviruses: kinetics, correlates of protection, and association with severity. Nat Commun 11, 4704 (2020). https://doi.org/10.1038/s41467-020-18450-4
- Magnetic Bead-Based Immunoassay Allows Rapid, Inexpensive, and Quantitative Detection of Human SARS-CoV-2 Antibodies. Luciano F. Huergo, Khaled A. Selim, Marcelo S. Conzentino, Edileusa C. M. Gerhardt, Adrian R. S. Santos, Berenike Wagner, Janette T. Alford, Nelli Deobald, Fabio O. Pedrosa, Emanuel M. de Souza, Meri B. Nogueira, Sônia M. Raboni, Dênio Souto, Fabiane G. M. Rego, Dalila L. Zanette, Mateus N. Aoki, Jeanine M. Nardin, Bruna Fornazari, Hugo M. P. Morales, Vânia A. Borges, Annika Nelde, Juliane S. Walz, Matthias Becker, Nicole Schneiderhan-Marra, Ulrich Rothbauer, Rodrigo A. Reis, and Karl Forchhammer ACS Sensors 2021 6 (3), 703-708 DOI: 10.1021/acssensors.0c02544
- Ecke, A., Westphalen, T., Hornung, J. et al. A rapid magnetic bead-based immunoassay for sensitive determination of diclofenac. Anal Bioanal Chem 414, 1563–1573 (2022). https://doi.org/10.1007/s00216-021-03778-7