Published June 26, 2023 | Version v1
Journal article Open

A brief focus on cell culture and its techniques

  • 1. 1 Mahatma Jyotiba Phule Rohilkhand University, Bareilly 2Indian Veterinary Research Institute, Izatnagar, Bareilly 3KIET group of Institutions Ghaziabad

Description

Cell culture involves a complex of processes of cell isolation from their natural environment (in vivo) and subsequent growth in a controlled environmental artificial condition (in vitro). Cells from specific tissues or organs are cultured as short term or established cell lines which are widely used for research and diagnosis, most specially in the aspect of viral infection, because pathogenic viral isolation depends on the availability of permissible cell cultures. Cell culture provides the required setting for the detection and identification of numerous pathogens of humans, which is achieved via virus isolation in the cell culture as the “gold standard” for virus discovery. 

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