Diet and the evolution of ADH7 across seven orders of mammals
Creators
- 1. University of Calgary
- 2. BE Bioinsight & Ecoa*
- 3. Guanacaste Conservation Area
- 4. Kids Saving the Rainforest Wildlife Rescue Center*
- 5. Sabah Parks
- 6. Kids Saving the Rainforest
- 7. Swansea University
- 8. Dartmouth College
- 9. Federal University of Rio Grande do Norte
- 10. BioTork*
Description
Dietary variation within and across species drives the eco-evolutionary responsiveness of genes necessary to metabolize nutrients and other components. Recent evidence from humans and other mammals suggests that sugar-rich diets of floral nectar and ripe fruit have favored mutations in, and functional preservation of, the ADH7 gene, which encodes the ADH class 4 enzyme responsible for metabolizing ethanol. Here we interrogate a large, comparative dataset of ADH7 gene sequence variation, including the amino acid residue located at key site 294 that regulates the affinity of ADH7 for ethanol. Our analyses span 171 mammal species, including 59 newly sequenced. We report extensive variation, especially among frugivorous and nectarivorous bats, with potential for functional impact, and widespread variation in the retention and probable pseudogenization of ADH7. However, we find little statistical evidence of a broad impact of diet on putative ADH7 function or tuning at site 294 across mammals, suggesting that the evolution of ADH7 is shaped by complex factors. Our study reports extensive new diversity in a gene of longstanding ecological interest, offers new sources of variation to be explored in functional assays in future studies, and advances our understanding of the processes of molecular evolution.
Notes
Funding provided by: Leakey Foundation
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100005966
Award Number:
Funding provided by: Canada Research Chairs
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100001804
Award Number: 950-231257
Funding provided by: David and Lucile Packard Foundation
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000008
Award Number: 2007-31754
Funding provided by: Natural Sciences and Engineering Research Council of Canada
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100000038
Award Number: RGPIN-2017-03782
Funding provided by: Sigma Xia
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100011084
Award Number:
Funding provided by: Operation Wallacea
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100021367
Award Number:
Funding provided by: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100002322
Award Number:
Funding provided by: Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100006162
Award Number: FACEPE IBPG-0660-2.04/14
Funding provided by: Alberta Innovates
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100009192
Award Number:
Files
ADH7_DietData_December2022.txt
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Additional details
Related works
- Is supplemented by
- https://www.ncbi.nlm.nih.gov/bioproject/PRJNA926128 (URL)