Dryad
Published June 22, 2023 | Version v1
Dataset Open

Landscape of Mast cell populations across organs in mice and humans

Creators

  • 1. Inserm
  • 2. University of Edinburgh
  • 3. Centre national de la recherche scientifique
  • 4. Uppsala University
  • 5. Johns Hopkins University
  • 6. Stanford University
  • 7. Singapore Immunology Network

Description

Mast cells (MCs) are tissue-resident immune cells which exhibit homeostatic and neuron-associated functions. Here we combined whole-tissue imaging and single-cell RNA sequencing datasets to generate a pan-organ analysis of MCs in mice and humans at steady state. In mice, we identify two mutually exclusive MC populations, MrgprB2+ connective tissue-type MCs and MrgprB2neg mucosal-type MCs, with specific transcriptomic core signatures. While MrgprB2+ MCs develop in-utero independently of the bone marrow, MrgprB2neg MCs develop after birth and are renewed by bone marrow progenitors. In humans, we unbiasedly identify 7 MC subsets (MC1 to 7) distributed across 12 organs with different transcriptomic core signatures. MC1 are preferentially enriched in the bladder, MC2 in the lungs, and MC4, MC6 and MC7 in the skin. Conversely, MC3 and MC5 are shared by most organs, but not skin. This comprehensive analysis offers valuable insights into the natural diversity of MC subtypes in both mice and humans.

Notes

Tar archives contain raw sequencing data in fastq format.

Rds file contains processed Seurat R objects.

Funding provided by: European Research Council
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100000781
Award Number:

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