Published December 18, 2023 | Version v1
Dataset Open

Tomopteris sp. Transcriptome data and associated annotations

  • 1. Bigelow Laboratory for Ocean Sciences

Description

Transcriptome sequences for specimen RAD2-051, Tompteris sp. 

Files are:

RAD2-051_ISOseq_clustered.hq.fasta --> Long read PacBio IsoSeq assembly of Tomopteris mRNA, from body segments

RAD2-051_combinedAssembly_c0.95_EXPRfilt_nocontam.fasta --> Combined Trinity and IsoSeq assemblies to capture all expressed genes in dorsal cirri and body segments. Contaminants removed by expression count level filtering and manual removal of human, drosophila, and xenopus sequences (100% blastn hits).

RAD2-051_combinedAssembly_c0.95_EXPRfilt_nocontam.fasta.transdecoder.pep --> translated proteins from the combined assembly file.

RAD2-051.tsv --> InterProScan annotation for RAD2-051 proteins.

RAD2-051.x.swissprot.BLASTXOUT-hssp.txt --> best BLASTX hits of RAD2-051 transcripts against the curated SwissProt database.

RAD2-051_Tomopteris.sp.counts.CPMs.csv --> file with read counts in CPMs for mRNA quantification for samples from dorsal cirri and body segments.  LA=Left antenna (dorsal cirrus); RA=Right antenna (dorsa cirrus); BD=body segment sample.

RAD2-051.iproscan.goa --> a gene ontology annotation file for RAD2-051 annotations from interproscan. For use with topGO in R.

 

 

 

 

Files

RAD2-051.x.swissprot.BLASTXOUT-hssp.txt

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