Cellular architecture of evolving inflammatory lesions and multiple sclerosis pathology
Creators
- 1. Stockholm University, Science for Life Laboratory, Department of Biophysics and Biochemistry, Stockholm, Sweden
- 2. Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Laboratory of Molecular Neurobiology, Biomedicum, Stockholm, Sweden
- 3. BioImage Informatics Facility, Science for Life Laboratory, SciLifeLab, Sweden
- 4. Karolinska Institutet, Department of Clinical Neuroscience, Center for Molecular Medicine, Karolinska University Hospital, Solna, Sweden
Description
Multiple sclerosis (MS) is a neurological disease characterized by multifocal lesions and smoldering pathology. While single-cell analyses provided insights into cytopathology, evolving cellular processes underlying MS remain poorly understood. We modeled the cellular dynamics of MS by examining temporal and regional rates of disease progression in mouse experimental autoimmune encephalomyelitis. By performing single-cell spatial expression profiling using In situ sequencing, we annotated disease neighborhoods during lesion evolution and found centrifugal propagation of active lesions. We demonstrated that disease-associated (DA)-glia arise independently of lesions and are dynamically induced and resolved over the disease course. Single-cell spatial mapping of human archival MS spinal cords confirmed the differential distribution of homeostatic and DA-glia, enabled deconvolution of active and inactive lesions into sub-compartments, and identified new lesion areas. By establishing a spatial resource of mouse and human MS neuropathology at a single-cell resolution, our study unveils the intricate cellular dynamics underlying MS evolution.
Files
CARE_models.zip
Files
(45.7 GB)
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