Aggregation mechanism and branched 3D morphologies of pathological human light chain proteins under reducing conditions

Here, we examined the aggregation mechanism and structures of the pathological human multiple myeloma light 
chain aggregates (hLC) after disrupting stabilizing disulfide bonds by various reducing agents. The aggregation 
kinetics were measured in the presence of three commonly used disulfide reducers (TCEP, DTT and glutathione), 
and the resulting aggregates were visualized by the combination of light and confocal/super-resolution STED 
microscopy. We find that aggregation kinetics can be described by two apparent macroscopic rate constants of 
the Finke-Watzky model related to the nucleation and the growth process. Surprisingly, the growth rate constants 
decreased at higher protein concentrations, which we interpret as the involvement of an aggregation active 
monomer particle that is successively depleted at high concentrations due to shifts in a monomer/dimer equilibrium. Seeding experiments demonstrated the specificity of the aggregates; only certain seeds accelerated the 
aggregation, while others eventually slowed down the aggregation. Three-dimensional visualization of the 
overall structures of the final aggregates at submicrometer resolution showed variable, reducer-specific branched 
morphologies with non-trivial fractal dimensions. Thus, the disruption of the stabilizing disulfide bonds in hLC 
leads to specific large, branched aggregates formed by the monomer-addition mechanism.