Application of Screen Printed Diamond Electrode, Coupled with "Point-of-Care" Platform, for Nanomolar Quantification of Phytonutrient Pterostilbene in Dietary Supplements: An Experimental Study Supported by Theory
Creators
- 1. Department of Analytical Chemistry, Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia
- 2. Scientific Institution, Institute of Chemistry, Technology and Metallurgy, National Institute University of Belgrade, 11000 Belgrade, Serbia
- 3. Institute for Oncology and Radiology of Serbia (IORS), Department of Radiotherapy Physics, Pasterova 14, 11000 Belgrade, Serbia
- 4. Department of Analytical Chemistry, Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia; Department for Ecology and Chemical Technology, South Ural State University, Lenin Prospect 76, 454080 Chelyabinsk, Russia
- 5. Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Radlinského 9, SK-812 37 Bratislava, Slovakia
Description
Abstract
Herein, a screen–printed diamond electrode (SPDE) coupled with a “point-of-care” platform (30 µL-drop concepts, single-drop-detection approach) was successfully applied for the electrochemical determination of pterostilbene (PTS). Cyclic voltammetry identified irreversible oxidation of PTS, where oxidation peak was shown to be strongly dependent on the pH of the working environmental. Although the proposition of the detailed electrochemical oxidation mechanism of PTS goes out of the scope of the present research, we have determined the most probable reactive site of our analyte, by utilizing DFT-based reactivity descriptors (Fukui functions). For electrochemical quantification of PTS, oxidation peak at 0.32 V (vs. Ag/AgCl) was followed in presence of 0.5 mol L−1 of Briton–Robinson buffer solution (pH = 9). Coupled with the optimized parameters of differential pulse voltammetry (DPV), SPDE detected PTS in two linear ranges (first range was from 0.011 to 0.912 µmol L−1; second range was from 0.912 to 4.420 µmol L−1), providing the LOD and LOQ on a nanomolar level (3.1 nmol L−1 and 10.0 nmol L−1, respectively). The selectivity of the optimized DPV method was found to be excellent, with the current changes of less than 7%, in the presence of ten times higher concentrations of the certain interferences. The practical applicability of the SPDE and single-drop-detection approach in dietary supplements (with a declared PTS content of 50 mg/tablet), with the recovery values ranging from 95 to 102%, shows that the developed method has high potential for precise and accurate PTS detection, as well as exceptional miniaturization possibilities of relevant equipment for on-site sensing.
Notes
Files
Application of Screen Printed Diamond Electrode, Coupled with Point of Care Platform for Nanomolar Quantification of Phytonutrient Pterostilbene in Dietary Supplements. An Experimental Study Supported by Theory.pdf
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Additional details
Related works
- Is identical to
- https://www.mdpi.com/2227-9040/11/1/15 (URL)
- Is part of
- 2227-9040 (ISSN)
Funding
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200168 (University of Belgrade, Faculty of Chemistry) 200168
- Ministry of Education, Science and Technological Development
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200026 (University of Belgrade, Institute of Chemistry, Technology and Metallurgy - IChTM) 200026
- Ministry of Education, Science and Technological Development