Published March 15, 2023
| Version v1
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20230315 ImageJ bio-format importer multi-channel ND2 bug
Description
A bug wherein ND2 images taken with different camera settings are read incorrectly by ImageJ's bio-format importer
imageJ_test.nd2 - The ND2 file produced by NIS Elements AR on a Nikon LV100 microscope. 11 time steps, 2 channels (images taken with different filter blocks).
test_image_ImageJ.jpg - A screenshot of Fiji/ImageJ after opening the ND2 with bio-formats importer. The number of dimensions is correct (11 time steps, two channels) but each channel is mono rather than an rgb image and the images are badly jumbled.
test_image_metadata_ImageJ.jpg - The OME metadata of the ND2.
test_image_movie_imageJ.avi - The ImageJ output exported as an AVI.
test_image_NIS.jpg - A screenshot of the ND2 opened in Nikon's NIS Elements Viewer.
Files
test_image_ImageJ.jpg
Files
(487.3 MB)
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