Published March 3, 2023 | Version v1
Dataset Open

Recombinant Expression and Purification of ABHD2 from E. coli

Creators

  • 1. SGC Toronto
  • 2. SGC Karolinksa
  • 3. SGC Karolinska

Description

α/β Hydrolase domain-containing protein 2 (ABHD2), is a member of the metabolic serine hydrolases superfamily & a progesterone membrane receptor. In sperm, it supports the activation of CatSper which leads to sperm hypermotility. ABHD2 is 425 aa and has a N-terminal transmembrane domain followed by an extracellular facing domain. Here we report the recombinant expression and purification of ABHD2 amino acids 33-435 from E. coli. To the best of our knowledge, we are the first to have recombinantly purified active ABHD2.

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