peterjc/thapbi-pict: THAPBI PICT v0.14.0

Released on PyPI on 2023-02-03:

https://pypi.org/project/thapbi-pict/0.14.0/

The tool now offers UNOISE style read-correction (off by default), either a built-in implementation of the published algorithm, or by invoking the command line tools USEARCH or VSEARCH. This algorithm requires access to all the reads prior to abundance level thresholds, and thus required some restructuring of the pipeline. The read-preparation step therefore only discards singletons, with the new sample-tally step combining all the unique sequence variants (ASVs). This can optionally apply read-correction before applying the abundance thresholds (which can still be set dynamically using control samples). This is output as a sequence tally table which can be converted to BIOM format. Furthermore, the classifier output now extends the sequence tally table with additional columns containing the taxid and genus-species.