Review on Next Generation Sequencing

This review examines the current state of Next-generation sequencing (NGS) technology, its applications, and its impact on scientific research. NGS allows for the simultaneous sequencing of millions of DNA fragments, making it a powerful tool for studying genetic variation and gene expression. The review discusses the advantages of NGS over traditional Sanger sequencing, including higher throughput, lower cost, and greater sensitivity. NGS has been widely used in many areas of research, including genomics, transcriptomics, epigenomics, metagenomics, and microbiology. The technology has enabled the discovery of new genes, identification of disease-causing mutations, and the characterization of complex biological processes. The review also highlights the challenges associated with NGS, such as the need for bioinformatics expertise and the generation of large amounts of data. However, the continued development and improvement of NGS technologies have led to the creation of new sequencing platforms, such as nanopore sequencing, which promise to further expand the range of applications of NGS. The review concludes that NGS has already had a profound impact on scientific research and will likely continue to do so in the future, providing new insights into the biology of organisms and enabling the development of personalized medicine.